Doctor en Ciencias Biológicas de la UBA
Investigador Adjunto CONICET.
Jefe de Trabajos Prácticos, Departamento de Fisiología, Biología Molecular y Celular, FCEN-UBA.
Posdoctorado en el National Institutes of Health (NIH), Bethesda, MD, USA. Bajo la dirección del Dr. Gordon L. Hager
Doctorado en el IFIBYNE-CONICET, bajo la dirección de la Dra. Adali Pecci
Especialidad: Endocrinología Molecular
Premio Estímulo 2019 de la Academia Nacional de Ciencias Exactas, Físicas y Naturales, en el área Ciencias Biológicas.
Reconocimiento a la Excelencia Académica 2022 por parte de la Universidad de Buenos Aires.
Miembro de la Academia Joven de Argentina, desde 2020.
Miembro de la Comisión Académica de control de gestión del Centro de Microscopías Gregorio Webber. Facultad de Ciencias Exactas y Naturales-UBA, desde 2022
Miembro de la Comisión de Carrera en Ciencias Biológicas (CCCB), Facultad de Ciencias Exactas y Naturales-UBA, desde 2024
Palabras clave: Receptor de Glucocorticoides; Factores de Transcripción, Receptores de esteroides, Microscopía de fluorescencia; oligomerización de proteínas; Número y Brillo; Seguimiento de moléculas únicas.
– Chromatin structure and dynamics: one nucleosome at a time. Diego M. Presman✉, Belén Benitez, Agustina Lafuente, Alejo Vazquez Lareu. Histochem Cell Biol (2024). https://doi.org/10.1007/s00418-024-02281-1
– Transcriptional Condensates: A Blessing or a Curse for Gene Regulation? Martin Stortz, Diego M. Presman✉, and Valeria Levi✉. Communications Biology 2024, 7:187. https://doi.org/10.1038/s42003-024-05892-5
– The mineralocorticoid receptor forms higher order oligomers upon DNA binding. Gregory Fettweis, Thomas A. Johnson, Brian Almeida-Prieto, Julián Weller-Pérez, Diego M. Presman, Gordon L. Hager✉, Diego Alvarez de la Rosa✉. Protein Sci. 2024 33(3):e4890. https://doi.org/10.1002/pro.4890
– Dynamic switching of transcriptional regulators between two distinct low-mobility chromatin states. Kaustubh Wagh†, Diana A Stavreva†, Rikke AM Jensen, Ville Paakinaho, Gregory Fettweis, R Louis Schiltz, Daniel Wüstner, Susanne Mandrup, Diego M Presman✉, Arpita Upadhyaya✉, Gordon L Hager✉. Science Advances 2023, 9:eade1122. †co-first authors. ✉corresponding authors. https://www.science.org/doi/10.1126/sciadv.ade1122
– SOX2 modulates the nuclear organization and transcriptional activity of the glucocorticoid receptor. Martin Stortz, Camila Oses, Camila Vázquez Echegaray, Adali Pecci, Alejandra Guberman, Diego M. Presman
and Valeria Levi. Journal of Molecular Biology 2022 Oct 26;167869. Online ahead of print. corresponding authors. https://doi.org/10.1016/j.jmb.2022.167869.
– The multivalency of the glucocorticoid receptor ligand-binding domain explains its manifold physiological activities. Alba Jiménez-Panizo#, Andrea Alegre-Martí#, Theophilus Tettey#, Gregory Fettweis, Montserrat Abella, Rosa Antón, Thomas A Johnson, Sohyoung Kim, R. Louis Schiltz, Israel Nuñez-Barrios, Joan Font-Díaz, Carme Caelles, Annabel F. Valledor, Paloma Pérez, Ana M. Rojas, Juan Fernández-Recio, Diego M. Presman, Gordon L. Hager, Pablo Fuentes-Prior, and Eva Estébanez-Perpiñá. #co-first authors. corresponding authors. Nucleic Acids Research, 2022; (50) 22:13063-13082. https://doi.org/10.1093/nar/gkac1119.
– Single-molecule tracking reveals two low-mobility states for chromatin and transcriptional regulators within the nucleus. Kaustubh Wagh†, Diana A Stavreva†, Rikke AM Jensen, Ville Paakinaho, Gregory Fettweis, R Louis Schiltz, Daniel Wüstner, Susanne Mandrup, Diego M Presman, Arpita Upadhyaya, Gordon L Hager. Disponible como pre-print en bioRxiv https://doi.org/10.1101/2022.07.25.501476.
– Genome-wide Binding Potential and Regulatory Activity of the Glucocorticoid Receptor’s Monomeric and Dimeric Forms. Thomas A. Johnson; Ville Paakinaho, Sohyoung Kim, Gordon L. Hager, and Diego M. Presman. Nature Communications 2021, 12, 1987. corresponding authors. https://doi.org/10.1038/s41467-021-22234-9.
– Power-law behaviour of transcription factor dynamics at the single-molecule level implies a continuum affinity model#. David A. Garcia*, Gregory Fettweis*, Diego M. Presman*, Ville Paakinaho, Christopher Jarzynski, Arpita Upadhyaya, and Gordon L. Hager. Nucleic Acids Research, 2021, gkab072. *co-first authors. #Destacado como “Breakthrough article”.
https://doi.org/10.1093/nar/gkab072.
– An intrinsically disordered region-mediated confinement state contributes to the dynamics and function of transcription factors. David A. Garcia, Thomas A. Johnson*, Diego M. Presman*, Gregory Fettweis, Kaustubh Wagh, Lorenzo Rinaldi, Diana A. Stavreva, Ville Paakinaho, Rikke A.M. Jensen, Susanne Mandrup, Arpita Upadhyaya, and Gordon L. Hager. Molecular Cell, 2021, 81:1-15. *co-second authors.
https://doi.org/10.1016/j.molcel.2021.01.013.
– Phasing the intranuclear organization of steroid hormone receptors. Martin Stortz*
, Diego M. Presman*
, Adali Pecci
, and Valeria Levi
. Biochemical Journal 2021, 478(2):443-461.
https://doi.org/10.1042/BCJ20200883 *Co-first authors;
corresponding authors.
– Unraveling the Molecular Interactions Involved in Phase Separation of Glucocorticoid Receptor. Martin Stortz, Adali Pecci, Diego Presman
, and Valeria Levi
. BMC Biology, 2020, 18(1):59.
https://doi.org/10.1186/s12915-020-00788-2.
co-corresponding authors
– Glucocorticoid Receptor Quaternary Structure Drives Chromatin Occupancy and Transcriptional Outcome. Ville Paakinaho, Thomas A. Johnson, Diego M. Presman
, Gordon L. Hager
. Genome Research, 2019 Aug;29(8):1223-1234.
https://doi.org/10.1101/gr.244814.118.
co-corresponding authors. Destacada en F1000.
– Assaying Homodimers of NF-κB in Live Single Cells. Erik W. Martin*, Sayantan Chakraborty*, Diego M. Presman, Francesco Tomassoni Ardori, Kyu-Seon Oh, Mary Kaileh, Lino Tessarollo, Myong-Hee Sung. Frontiers Immunology 2019, Nov 07 10, 2609.
https://doi.org/10.3389/fimmu.2019.02609.
– The Glucocorticoid Receptor Interferes with Progesterone Receptor-Dependent Genomic Regulation in Breast Cancer Cells. Maria Florencia Ogara, Santiago A. Rodríguez-Seguí, Melisa Marini, A. Silvina Nacht, Martin Stortz, Valeria Levi, Diego M. Presman, Guillermo P. Vicent and Adali Pecci. Nucleic Acid Research 2019, Oct 10. pii: gkz857.
https://doi.org/10.1093/nar/gkz857.
– Meta-analysis of Chromatin Programming by Steroid Receptors. Ville Paakinaho, Erin E. Swinstead, Diego M. Presman, Lars Grøntved, and Gordon L. Hager. Cell Reports 2019, Sept 24 28(13): 3523-3534.
https://doi.org/10.1016/j.celrep.2019.08.039
– Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility. Diana A. Stavreva, David A. Garcia, Gregory Fettweis, Prabhakar R. Gudla, George F. Zaki, Vikas Soni, Andrew McGowan, Geneva Williams, Anh Huynh, Murali Palangat, R. Louis Schiltz, Thomas A. Johnson, Diego M. Presman, Matthew L. Ferguson, Gianluca Pegoraro, Arpita Upadhyaya, and Gordon L. Hager. Molecular Cell, 2019 Jul 26. pii: S1097-2765(19)30499-X.
https://doi.org/10.1016/j.molcel.2019.06.042.
– Single-cell resolution and quantitation of targeted glucocorticoid delivery in the thymus. Matthew D. Taves, Paul R. Mittelstadt, Diego M. Presman, Gordon L. Hager, Jonathan D. Ashwell. Cell Reports, 2019, 26(13):3629-3642.e4.
https://doi.org/10.1016/j.celrep.2019.02.108.
– 21-hydroxy-6,19-epoxyprogesterone: a Promising Therapeutic Agent and a Molecular Tool for Deciphering Glucocorticoid Action. Pecci, A, Alvarez, L.D., Presman, D.M., Burton, G. Minireviews in Medicinal Chemistry 2018. 18(5):428-438.
https://doi.org/10.2174/1389557516666160118112313.
– Single-molecule Analysis of Glucocorticoid Receptor and Cofactor Action in Living Cells. Paakinaho, V*., Presman, D.M*., Ball, D., Johnson, T.A., Schiltz, R.L., Levitt, P., Mazza, D., Morisaki, T., Karpova, T.S., Hager, G.L. Nature Communications 2017, Jun 21;8:15896.
https://doi.org/10.1038/ncomms15896. *co-first authors. Destacada en F1000.
– Quantifying Transcription Factor Binding Dynamics at the Single-molecule Level in Live Cells. Presman, D.M*., Ball, D*., Paakinaho, V*., Grimm, J.B., Lavis, L.D. Karpova, T.S., Hager, G.L. Methods 2017. pii: S1046-2023(16)30450-9.
https://doi.org/10.1016/j.ymeth.2017.03.014. *co-first authors.
– Molecular dynamics simulations of the glucocorticoid receptor DNA-binding domain suggest a role of the lever-arm mobility in transcriptional output. Álvarez LD, Presman DM, Pecci A. PLoS One. 2017 Dec 15;12(12):e0189588.
https://doi.org/10.1371/journal.pone.0189588.
– Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape. Stortz M, Presman DM, Bruno L, Annibale P, Dansey MV, Burton G, Gratton E, Pecci A, Levi V. Sci Rep. 2017, 7(1):6219.
https://doi.org/10.1038/s41598-017-06676-0.
– Transcription factor assisted loading and enhancer dynamics dictate the fasting response. Goldstein, I., Baek, S., Presman, D.M., Paakinaho, V., Swinstead, E.E, Hager, G.L. Genome Research, 2017. (3):427-439.
https://doi.org/10.1101/gr.212175.116.
– DNA-binding Triggers Tetramerization of the Glucocorticoid Receptor in Live Cells. Presman, D.M
, Ganguly, S., Schiltz, R.L., Johnson, T.A., Karpova, T.S., Hager, G.L
. Proc Natl Acad Sci USA, 2016 113 (29) 8236-8241.
co-corresponding authors.
https://doi.org/10.1073/pnas.1606774113– Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions. Swinstead, E.E., Miranda, T.B., Paakinaho, V., Baek, S., Goldstein, I., Hawkins, M., Karpova, T.S., Ball, D., Mazza, D., Lavis, L.D., Grimm, J.B., Morisaki, M., Grøntved, L., Presman, D.M., and Hager, G.L. Cell, 2016, 165:593-605. Destacada en F1000.
https://doi.org/10.1016/j.cell.2016.02.067
– Pioneer factors and ATP-dependent chromatin remodeling factors interact dynamically: A new perspective. Swinstead, E.E., Paakinaho, V., Presman, D.M., Hager, G.L. Bioessays 2016, 38(11):1150-1157.
https://doi.org/10.1002/bies.201600137
– Role of 3′-5′-cyclic adenosine monophosphate on the epidermal growth factor dependent survival in mammary epithelial cells. Grinman, D.Y*, Romorini, L*., Presman, D.M., Rocha-Viegas, L., Coso, O.A., Davio, C., Pecci, A. Mol Cell Endocrinol. 2015. 419:259-67. *co-first author.
https://doi.org/10.1016/j.mce.2015.10.026
– Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP. Muratcioglu, S., Presman, D.M*, Pooley, J.R*, Grøntved, L., Hager, G.L., Nussinov, R., Keskin, O., Gursoy, A. Biophys J. 2015; 109(6):1227-39. *co-second authors. Destacada como “Best of 2015 re-print issue of Biophysical Journal”.
https://doi.org/10.1016/j.bpj.2015.06.044
– Live cell imaging unveils multiple domain requirements for in vivo dimerization of the glucocorticoid receptor. Presman, D.M., Ogara, M.F, Stortz, M., Alvarez, L.D., Pooley, J.R., Schiltz, R.L, Grøntved, L., Johnson, T.A., Mittelstadt, P.R., Ashwell, J.D., Ganesan, S., Burton, G., Levi, V., Hager, G.L., Pecci, A. PLoS Biology 2014 ;12(3):e1001813. Destacada en F1000.
https://doi.org/10.1371/journal.pbio.1001813
– Melatonin inhibits glucocorticoid-dependent GR-TIF2 interaction in newborn hamster kidney (BHK) cells. Presman, D.M., Levi, V., Pignataro, O.P., Pecci, A. Mol Cell Endocrinol. 2012; 349(2):214-21.
https://doi.org/10.1016/j.mce.2011.10.026
– Insights on glucocorticoid receptor activity modulation through the binding of rigid steroids. Presman, D.M, Alvarez, L.D., Levi, V., Eduardo, S., Digman, M.A., Martí, M.A., Veleiro, A.S., Burton, G., Pecci, A. PLoS One. 2010; 5(10):e13279.
https://doi.org/10.1371/journal.pone.0013279
– Exploring the molecular basis of action of the passive antiglucocorticoid 21-hydroxy-6,19-epoxyprogesterone. Alvarez, L.D., Martí, M.A., Veleiro, A.S., Presman, D.M., Estrin, D.A., Pecci, A., Burton, G. J Med Chem. 2008; 51(5):1352-60.
https://doi.org/10.1021/jm800007w
– Melatonin inhibits glucocorticoid receptor nuclear translocation in mouse thymocytes. Presman, D.M., Hoijman, E., Ceballos, N.R., Galigniana, M.D., Pecci, A. Endocrinology. 2006; 147(11):5452-9.
https://doi.org/10.1210/en.2006-0252
